Cryoscopic Determination of Molecular Weight

IntroductionMany people, including me way back when, do
Freezing point depression is a colligative propertyfreezing point the hard way. By this I mean that we
meaning that the amount of change in the freezingfabricated our own apparatus with test tubes, glass
point depends only on the number of particlesthermometers, a piece of wire, and an acetone plus
dissolved in the solution. The freezing point depressiondry ice bath. We placed our sample into the tube,
does not care what the particles are; it is onlyimmersed the thermometer with the wire looped
concerned with how many are there. Because of this,around it then sticking out as a handle, and then froze
the measurement of the change in freezing pointthe solution in the dry ice acetone mixture. Once
between a pure solvent, and a solvent with a knownfrozen, we removed our apparatus and painstakenly
amount of substance in it gives an accurate, andplotted the rise in temperature moving the wire up and
absolute, value of the number of molecules dissolved indown to keep the temperature constant. With this plot
the solution. Knowing the mass of the solute and thewe estimated freezing point the best we could,
freezing point of the pure solvent provides enoughcleaned our device, and went on to the next sample.
information to accurately calculate the Molecules perThe process was slow, kind of fun, but fairly inacurate
unit mass of the solute.and not very cost effective for a commercial lab.
DiscussionEnter the cryoscope; an instrument that does all this,
Each solvent has its own freezing point and with it, itsautomatically and on small samples at that. With
own molal freezing - point constant. The molal freezingmicroliter size samples up to a milliliter or so, a
point constant is the depression in degrees Centigradecryoscope accurately measures freezing point
of the solvent when 1 mole of solute is dissolved in 1depression to thousandths of a degree centigrade on
kilogram of the solvent (Chemical Technician's Readya calibrated, basically push button instrument. A single
Reference Handbook, pg 435). Freezing pointvalue is returned from which molecular weight is easily
constants are published values available for most purecalculated. Hazardous solvents, such as benzene, can
solvents.readily be used with little risk of analyst exposure.