Uses of Deionised Water in Laboratory Setting

One of the most important uses of deionised water isinside the microbiology lab and every process must be
in the laboratory. May it be a chemical, microbiological,done asceptically.
genetics and molecular biology, medical or tissueIn a molecular biology lab, deionised water needs to
culture laboratory, water is an indispensable item insideundergo further filtration to be DNase or RNase-free
the lab.to be deemed usable in this setting. Molecular biology
These are areas of science which call for the one ofand genetic experiments are particularly sensitive to
purest form of water available known as deionisedimpurities because of the need for an accurate and
water.exact set of results. The standard error must be very
The process involved in deionising water is long andslim indeed.
painstaking, ensuring that the end product isThe same goes for a tissue culture laboratory,
uncontaminated and free from impurities. It undergoeseverything must be sterile and the processes must be
a five step process of filtration: organic trap, twin bedasceptic. Deionised water is also used in media
deionizer, mixed bed resin, ultra violet treatment, andpreparation as well as in the set-up of the needed
filtration through a 0.02 micrometer pore size.items and equipment.
Organic impurities are filtered out first then the ionsA medical laboratory processes clinical specimen of
(cations and anions) are removed. At this point, waterpatients to obtain more information on his/her health.
is deionised. However, there are still other impuritiesThe physician can then make a better evaluation of
found in water and so water is subjected to anthe patient's health based on the results of a urine
ultraviolet treatment to kill microorganisms. Anothersample or a blood sample, for instance. Therefore,
filtration through a mixed bed resin is used to removecareful handling and preparation of the samples and
any remaining impurities. Finally, water is filtered using athe whole test are performed to ensure that the
filter of a 0.02 pore size.results are accurate. The proper diagnosis of a
The end product is ideal for use in the laboratorypatient's health condition lay heavily on the results
wherein every single item must be in their purest formthese tests will show.
to come up with accurate results.Deionised water, however, must meet the standards
In the chemistry laboratory, it is used in preparing glassfor each type of laboratory since each also has a
wares, utensils, and other tools. Normal or tap waterdifferent need. Nonetheless, deionised water is already
usually has impurities present, such as fluorine andof a higher grade than tap water and it can be used
chlorine, which can influence the results of yourfor various laboratory preparations and procedures.
chemistry experiments.Aside from its crucial uses in the laboratory setting,
Deionised water is used to rinse glass wares,deionised water is also used extensively in the food
laboratory tools, as well as small and large apparatusprocessing, pharmaceutical, cosmetics, electronics, and
used in the microbiological testing. It is also used as aautomotive industries. It has even found its use into
solvent in media preparation and other microbiologicalyour local carwash. Water is indeed a central
procedures requiring water. Everything must be sterileingredient in everything we do.